The dilutions of a solution of known concentration were used to determine the concentration of unknown. Our data on CMCase activities of twelve enzyme preparations determined with two different RS assays Table 1 are very similar to those reported by Breuil and Saddler [ 11 ], who observed moderate overestimations of the endoglucanase CMCase activity of Trichoderma harzianum culture filtrates by the DNS assay in comparison with the NS assay.
By using pipette, 16 mL of distilled water was added into the test tubes accurately to make a volume of 20 mL. This involves the oxidation of the aldehyde functional group present in, for example, glucose and the ketone functional group in fructose.
At the same time, polygalacturonic acid, free of the methyl ester groups, can be used as a substrate in determination of the polygalacturonase activity with an RS assay. The activity values obtained with the DNS assay were 3- to 8-fold higher than those obtained with the NS assay. Different reducing sugars generally yield different color intensities; thus, it is necessary to calibrate for each reducing sugar.
Reducing value methods for maltodextrins. As a consequence, carboxymethyl cellulose can affect the calibration curve by enhancing the intensity of the developed color. In the same test tube 10ml of 1.
In particular, cellulase complexes produced by Trichoderma sp. In using enzymatic hydrolysis however, pre—treatment is necessary to open up the structure and to provide access for the enzyme to the active sites.
All test tubes were heated in boiling water bath for 5 minutes to allow reaction between glucose and DNS to occur. Nevertheless, examples of the questionable use of the DNS assay for measuring pectinase activity may be found in the scientific literature [ 1617 ]. In this case, the interpretation of experimental data obtained by both methods should not cause ambiguities.
Notes on sugar determination. However, the DNS assay gives 3- to 6-fold overestimations of xylanase activity against glucuronoxylan compared to the NS assay. The investigated parameters linearity, limit of detection, limit of quantification, precision and accuracy confirm that this method is adequate, reliable and suitable for the study of Reducing Sugar in food, beverage and drug samples.Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar - Free download as PDF File .pdf), Text File .txt) or read online for free.
Scribd is the 5/5(25). The determination of lactose in dairy product is important and there are The DNS method for estimating the concentration of reducing sugars in a sample Reducing sugars contain free carbonyl group, have the property to reduce many of the reagents. Determination of Reducing Sugars Using Dns Essay INTRODUCTION Determining the sugar concentration of food samples is very important especially in industries where quality control is monitored.
Reducing sugars concentrations were determined by spectrophotometry using the 3, 5 dinitrosalicylic acid (DNS) method. The first sample was operated as blank for zero.
The stock solution was prepared in order to find the unknown concentration of carbohydrate cereals powder, jam (total sugar content) and jams (reducing sugar content). Determination of Redusing Sugar Using Dns Method.
The production of reducing sugars by acid of carbohydrate cereals powder, jams (total sugar content) and jams (reducing sugar content) were performed in order to study reducing sugar production.
May 26, · Most of the methods for determination of carbohydrase activity are based on the analysis of reducing sugars (RSs) formed as a result of the enzymatic scission of the glycosidic bond between two carbohydrates or between a carbohydrate and a noncarbohydrate moiety.Download